2026-10-21 名古屋大学
＜関連情報＞
- https://www.nagoya-u.ac.jp/researchinfo/result/2026/10/1-73.html
- https://www.cell.com/cell-reports-physical-science/fulltext/S2666-3864(26)00519-9
単一小胞におけるマイクロRNAと膜タンパク質の非破壊的な共局在センシングにより、空間的な炎症シグネチャが明らかになる Non-destructive colocalization sensing of microRNAs and membrane proteins in single vesicles reveals spatial inflammation signatures
Shota Kawaguchi ∙ Taiga Ajiri S ∙ Kunanon Chattrairat ∙ … ∙ Kaoru Murakami ∙ Masaaki Murakami ∙ Takao Yasui
Cell Reports Physical Science Published:October 17, 2026
DOI:https://doi.org/10.1016/j.xcrp.2026.103613
Highlights
- Cellulose nanofiber sheets mediate fusion for single-extracellular vesicle (EV) sensing
- Inflammatory stimulation remodels proteins and microRNAs in tissue-derived EVs
- Single-EV colocalization identifies tissue-specific inflammatory EV populations
Summary
Chronic inflammation underlies a broad spectrum of diseases, yet current liquid biopsy approaches lack the spatial resolution and molecular specificity to detect localized, disease-specific inflammatory signals. Extracellular vesicles (EVs) offer a promising alternative, as they carry both membrane proteins and intravesicular biomolecules that reflect the physiological state and tissue of origin. Here, we present a sensing platform for single-vesicle colocalization analysis of membrane proteins and microRNAs (miRNAs), using cellulose nanofiber-mediated membrane fusion to non-destructively deliver probes into intact EVs. This approach enables simultaneous detection of tissue-specific and inflammation-associated markers in individual EVs. In kidney transplant recipients with chronic active antibody-mediated rejection, we identify EVs co-expressing the renal marker DPYS and miR-1246. In rheumatoid arthritis patients, we observe elevated levels of miR-1246+/CD81+ EVs in plasma. Our platform distinguishes among inflammation-related EV subpopulations that are undetectable using bulk or surface-only analyses, thereby offering a non-invasive strategy for localized inflammation profiling.
