2023-08-28 カリフォルニア工科大学(Caltech)
◆カリフォルニア工科大学の研究者は、RNAを「読む」だけでなく「書く」ことも可能な新しいシステムを開発。このシステムにより、細胞内のRNAの変化を時間とともに追跡することが可能になり、細胞プロセスの理解が進む。さらに、このシステムは他の細胞にRNAを運び込み治療することもでき、将来的には医療分野に新たな治療戦略をもたらす可能性がある。
<関連情報>
- https://www.caltech.edu/about/news/a-synthetic-rna-export-system-reveals-the-dynamic-lives-of-cells-and-suggests-direction-for-new-therapeutics
- https://www.cell.com/cell/fulltext/S0092-8674(23)00689-X
生きている細胞の計測と操作のためにRNA輸送を工学的に行う Engineering RNA export for measurement and manipulation of living cells
Felix Horns,Joe A. Martinez,Chengcheng Fan,Mehernaz Haque,James M. Linton,Victoria Tobin,Leah Santat,Ailiena O. Maggiolo,Pamela J. Bjorkman,Carlos Lois,Michael B. Elowitz
Cell Published:July 11, 2023
DOI:https://doi.org/10.1016/j.cell.2023.06.013
Highlights
•RNA exporters package and secrete RNA within protective nanoparticles
•Engineered exporters achieve high specificity for cargo RNA
•Sequencing exported RNA barcodes enables monitoring of cell population dynamics
•Exporters enable cell-to-cell delivery and expression of mRNA cargo
Summary
A system for programmable export of RNA molecules from living cells would enable both non-destructive monitoring of cell dynamics and engineering of cells capable of delivering executable RNA programs to other cells. We developed genetically encoded cellular RNA exporters, inspired by viruses, that efficiently package and secrete cargo RNA molecules from mammalian cells within protective nanoparticles. Exporting and sequencing RNA barcodes enabled non-destructive monitoring of cell population dynamics with clonal resolution. Further, by incorporating fusogens into the nanoparticles, we demonstrated the delivery, expression, and functional activity of exported mRNA in recipient cells. We term these systems COURIER (controlled output and uptake of RNA for interrogation, expression, and regulation). COURIER enables measurement of cell dynamics and establishes a foundation for hybrid cell and gene therapies based on cell-to-cell delivery of RNA.
