2024-03-13 デューク大学(Duke)
<関連情報>
- https://pratt.duke.edu/news/water-droplet-centrifuge/
- https://www.science.org/doi/10.1126/sciadv.adm8597
ヌクレオチド検出のためのエクソソームの音響分離と濃縮: ASCENDx Acoustic separation and concentration of exosomes for nucleotide detection: ASCENDx
TY D. NAQUIN , AIDAN J. CANNING , YUYANG GU , JIANING CHEN , […], AND TONY JUN HUANG
Science Advances Published:8 Mar 2024
DOI:https://doi.org/10.1126/sciadv.adm8597
Abstract
Efficient isolation and analysis of exosomal biomarkers hold transformative potential in biomedical applications. However, current methods are prone to contamination and require costly consumables, expensive equipment, and skilled personnel. Here, we introduce an innovative spaceship-like disc that allows Acoustic Separation and Concentration of Exosomes and Nucleotide Detection: ASCENDx. We created ASCENDx to use acoustically driven disc rotation on a spinning droplet to generate swift separation and concentration of exosomes from patient plasma samples. Integrated plasmonic nanostars on the ASCENDx disc enable label-free detection of enriched exosomes via surface-enhanced Raman scattering. Direct detection of circulating exosomal microRNA biomarkers from patient plasma samples by the ASCENDx platform facilitated a diagnostic assay for colorectal cancer with 95.8% sensitivity and 100% specificity. ASCENDx overcomes existing limitations in exosome-based molecular diagnostics and holds a powerful position for future biomedical research, precision medicine, and point-of-care medical diagnostics.
