汗の複数バイオマーカーをリアルタイム監視する新規ナノザイムを開発（Novel Nanozyme Powers Real-time Monitoring of Multiple Sweat Biomarkers）

2026-06-12 中国科学院（CAS）

Structural design of the enzyme-nanoenzyme cascade system and its practical applications. (Image by LIN Dan)

＜関連情報＞

• https://english.cas.cn/newsroom/research-news/202606/t20260605_1161141.shtml
• https://www.sciencedirect.com/science/article/abs/pii/S0956566326004586

カスケード増幅型2次元超薄型六角形CuドープLDOナノザイムを用いた、汗代謝物の多重ウェアラブルモニタリング Cascade-amplified 2D ultrathin hexagonal Cu-doped LDO nanozyme for multiplexed wearable monitoring of sweat metabolites

Ruoxuan Jiang, Anqi Liu, Qipeng Zhang, Lei Pan, Shudong Zhang, Dan Lin, Liang Yang, Changlong Jiang
Biosensors and Bioelectronics  Available online: 20 May 2026
DOI:https://doi.org/10.1016/j.bios.2026.118826

Abstract

Sweat-based metabolic monitoring offers a non-invasive alternative to blood tests, but its clinical utility is limited by the limited catalytic efficiency of current nanozyme sensors. Bimetallic nanozymes, with their intermetallic synergy, hold promise to overcome this limitation, yet rational design strategies remain underexplored. Herein, we report a Cu-doped Co-Al layered double oxide (Co₂AlCu_0.7 LDO) with an ultrathin hexagonal nanosheet morphology that exhibits exceptional peroxidase-like activity (Km = 0.30 mM, Vmax = 2.932 × 10⁻⁶ M s⁻¹). Mechanistic studies reveal that the intrinsic Co-Al bimetallic framework provides a synergistic electronic basis, while Cu doping optimizes the d-band center of metal active sites and introduces abundant oxygen vacancies, which act as synergistic active centers to facilitate H₂O₂ activation and dramatically enhance peroxidase-like activity. By immobilizing biomarker-specific oxidases onto the LDO nanosheets, we constructed an enzyme-nanozyme cascade reactor for sensitive detection of cholesterol, uric acid, and glucose. For point-of-care translation, this reactor was integrated into a colorimetric sponge coupled with smartphone imaging and machine learning, yielding a portable platform and a conceptual wristwatch for non-invasive sweat analysis. This work not only provides novel design strategies for high-performance bimetallic nanozymes but also expands its potential as advanced functional biomaterials for next-generation biosensing platforms of clinical diagnostics and therapeutic monitor.