2026-04-17 理化学研究所
本研究の概要
＜関連情報＞
- https://www.riken.jp/press/2026/20260417_3/index.html
- https://www.nature.com/articles/s41467-026-71371-6
CaMPARI-seqを用いた分子レベルおよび機能レベルの解析により、視覚フロー依存性行動を分離するための神経組織が明らかになった Molecular and functional dissection using CaMPARI-seq reveals the neuronal organization for dissociating optic flow-dependent behaviors
Koji Matsuda,Chung-Han Wang,Hisaya Kakinuma,Atsushi Toyoda,Tomoya Shiraki,Koichi Kawakami & Fumi Kubo
Nature Communications Published:17 April 2026
DOI:https://doi.org/10.1038/s41467-026-71371-6
Abstract
Optic flow processing is critical for the visual control of body and eye movements in many animals. Rotational and translational binocular optic flow patterns need to be clearly distinguished to induce different behavior outputs. However, the specific neuron types and their connectivity involved in this computation remain unclear. Here, we developed a method to link the functional labeling using a photoconvertible calcium indicator called CaMPARI2 and single-cell RNA sequencing (CaMPARI-seq) to investigate the transcriptional profile of the pretectum, a center for processing optic flow in larval zebrafish. Using this technique, we identified a pretectal cluster expressing tcf7l2, which can be further classified into molecularly distinct subclusters. In vivo calcium imaging and cell ablation revealed that nkx1.2lb-positive pretectal neurons are commissural inhibitory neurons required for the optomotor response but not for the optokinetic response. Our genetic and functional dissection using CaMPARI-seq uncovered the neuronal organization essential for dissociating different optic flow-dependent behaviors.
