2026-05-14 九州大学
PPR-DYWと標的RNAの複合体の立体構造 C塩基をU塩基に編集する様子
＜関連情報＞
- https://www.kyushu-u.ac.jp/ja/researches/view/1479
- https://www.nature.com/articles/s41467-026-72391-y
PPR-DYWタンパク質による植物オルガネラのCからUへのRNA編集の構造的基盤 Structural basis of plant organelle C-to-U RNA editing by PPR-DYW proteins
Takamasa Teramoto,Ryota Urushihara,Reiya Aoyama,Ayumi Okada,HMizuho Ichinose,Yusuke Yagi,Takahiro Nakamura,Bernard Gutmann & Yoshimitsu Kakuta
Nature Communications Published:27 April 2026
DOI:https://doi.org/10.1038/s41467-026-72391-y Unedited version
Abstract
Plants possess a unique C-to-U RNA editing mechanism mediated by PPR-DYW proteins, wherein the PPR domain recognizes specific RNA sequences while the DYW deaminase domain precisely edits the target C base—a process essential for functional protein expression in plant chloroplasts and mitochondria. The coordination of these two domains is considered crucial for precise RNA editing. In nature, this site-specific and precise base editing by PPR-DYW proteins distinguishes them from other base-editing deaminases. However, the absence of structures containing both PPR and DYW domains has limited our understanding of the precise RNA-editing mechanism of PPR-DYW proteins. Here, we present crystal structures of the consensus PPR-DYW (consPPR-DYW) protein, a representative of the PPR-DYW proteins, in both RNA-free and target RNA-bound states. Comparison between these states demonstrates domain movements upon target RNA binding, whereby the PPR domain accommodates the upstream sequence of the target C base in the proper conformation for editing while the DYW domain is optimally positioned for precise C-to-U conversion. These results, combined with comprehensive biochemical analyses, provide the foundation for a mechanistic model that explains the coordinated action of the PPR and DYW domains in achieving precise C-to-U editing.
