2022-12-07 カリフォルニア大学リバーサイド校(UCR)
線虫ではELT-3因子はストレス反応に関与することが知られており、その機能は他の2つの遺伝子、END-1とEND-3の機能に置き換わっている。ELT-3を欠失させたC.angariaと同様に、END-1とEND-3の両方が欠落した場合、線虫の腸は形成されなくなる。
<関連情報>
- https://news.ucr.edu/articles/2022/12/07/how-do-worms-develop-their-gut
- https://journals.biologists.com/dev/article/149/21/dev200984/278466/The-GATA-factor-ELT-3-specifies-endoderm-in
GATA因子ELT-3は、Caenorhabditis angariaの内胚葉を祖先遺伝子ネットワークで規定する The GATA factor ELT-3 specifies endoderm in Caenorhabditis angaria in an ancestral gene network
Gina Broitman-Maduro,Simo Sun,Taisei Kikuch,Morris F. Maduro
Development Published:24 October 2022
DOI:https://doi.org/10.1242/dev.200984
ABSTRACT
Endoderm specification in Caenorhabditis elegans occurs through a network in which maternally provided SKN-1/Nrf, with additional input from POP-1/TCF, activates the GATA factor cascade MED-1,2→END-1,3→ELT-2,7. Orthologues of the MED, END and ELT-7 factors are found only among nematodes closely related to C. elegans, raising the question of how gut is specified in their absence in more distant species in the genus. We find that the C. angaria, C. portoensis and C. monodelphis orthologues of the GATA factor gene elt-3 are expressed in the early E lineage, just before their elt-2 orthologues. In C. angaria, Can-pop-1(RNAi), Can-elt-3(RNAi) and a Can-elt-3 null mutation result in a penetrant ‘gutless’ phenotype. Can-pop-1 is necessary for Can-elt-3 activation, showing that it acts upstream. Forced early E lineage expression of Can-elt-3 in C. elegans can direct the expression of a Can-elt-2 transgene and rescue an elt-7 end-1 end-3; elt-2 quadruple mutant strain to viability. Our results demonstrate an ancestral mechanism for gut specification and differentiation in Caenorhabditis involving a simpler POP-1→ELT-3→ELT-2 gene network.
