2025-06-19 中国科学院(CAS)
Changes in microbial diversity and community structure during degradation of acetochlor by consortium AT1 (Image by DAI Yumeng)
<関連情報>
- https://english.cas.cn/newsroom/research_news/earth/202506/t20250619_1045824.shtml
- https://www.sciencedirect.com/science/article/abs/pii/S0301479725018687
微生物コンソーシアムAT1によるアセトクロルの生分解:マイクロコズムを中心とした微生物メタボロミクス機構と環境修復の実現可能性 Biodegradation of acetochlor by microbial consortium AT1: microcosm centric microbiomic-metabolomics mechanisms and environmental remediation feasibility
Yumeng Dai, Xinyu Guan, Zhiyang Han, Xu Li, Xiujuan Wang, Zhencheng Su, Huiwen Zhang, Xiang Li, Mingkai Xu
Journal of Environmental Management Available online: 24 May 2025
DOI:https://doi.org/10.1016/j.jenvman.2025.125892
Highlights
- Enrichment of degradation consortium AT1 from acetochlor-contaminated soil.
- AT1 exhibited tolerance and degradation capability for high doses of acetochlor.
- AT1 showed microbial structural and functional changes during acetochlor degradation.
- AT1 degraded acetochlor via novel pathways, producing various phenolic metabolites.
- AT1 had the ability to biodegrade acetochlor in soil environment.
Abstract
The excessive use of herbicide acetochlor (ACT) threatens crop health and the environment, necessitating effective remediation strategies. This study focused on a consortium named AT1, enriched from ACT-contaminated soil. Under optimized conditions (25 °C, pH 7, 1 % inoculum), AT1 almost completely degraded ACT (50–1000 mg/L) within 6–12 days. High-throughput sequencing of 16S rRNA gene revealed a reduction in community diversity over time, with Sphingomonas (58.6 %) and Diaphorobacter (26.43 %) as dominant taxa. A structure model and network analysis indicated strong microbial competition during the peak degradation. Predicted functions and liquid chromatography-mass spectrometry based metabolomics data identified benzene ring intermediates during ACT degradation, including 2,6-dimethylaniline, resorcinol, phenol, 3-ethyl-1,2-benzenediol, 1,2,3-trihydroxybenzene, phloroglucinol, and benzene-1,2,4-triol. Joint omics analysis revealed that AT1 likely degrades ACT via N-dealkylation by Pseudomonas, amide bond hydrolysis by Diaphorobacter, and carboxylation and hydroxylation by Sphingomonas, leading to the formation of these intermediate metabolites. Moreover, AT1 efficiently degraded key intermediates, particularly 2,6-dimethylaniline, phenol, and resorcinol, further enhancing ACT mineralization. Notably, AT1 efficiently degraded ACT in soil, resulting in a significant decrease in ACT environmental residues. These findings provide valuable insights for the discovery and identification of herbicide-degrading bacterial resources and the metabolic transformation of herbicides, and developing approaches for pollution control and biodegradation of amide herbicides.
