2025-06-19 中国科学院(CAS)
Effect of exogenous 2KGA on ascorbic acid (ASA), total phenolics (TP), and total flavonoids (TF) in Brassica campestris ssp (Image by GAO Mingffu)
<関連情報>
- https://english.cas.cn/newsroom/research_news/earth/202506/t20250619_1045826.shtml
- https://www.sciencedirect.com/science/article/abs/pii/S0981942825006072
L-アスコルビン酸生合成における2-ケト-L-グロン酸の介入は植物の炭素代謝と二次代謝産物蓄積に影響する Intervention of 2-keto-L-gulonic acid in L-ascorbic acid biosynthesis affects plant carbon metabolism and secondary metabolite accumulation
Mingfu Gao, Hao Sun, Weichao Yang, Hui Xu
Plant Physiology and Biochemistry Available online: 26 May 2025
DOI:https://doi.org/10.1016/j.plaphy.2025.110079
Highlights
- A dose-effect between ascorbate (ASA) accumulation and 2-keto-gulonate (2KGA) dose.
- High expression of ASA synthetase GLO is crucial for 2KGA-enhanced ASA accumulation.
- The elevated phenolics and flavonoids were positively correlated with ASA content.
- The TCA cycle, amino acid metabolism and photosynthesis were regulated by 2KGA
- Thirty transcription factors were differentially expressed.
Abstract
The role of 2-keto-L-gulonic acid (2KGA), a downstream product of the ascorbic acid (ASA) metabolic pathway, in the biosynthesis of plant bioactive compounds remains to be elucidated. In this study, we investigated the effects of exogenous 2KGA on ASA biosynthesis and secondary metabolite accumulation in the non-heading Chinese cabbage (Brassica campestris [syn. B. rapa] ssp. chinensis cv. Shanghai bok choy). The findings revealed a dose-dependent relationship between ASA accumulation in plants and 2KGA dose, with high expression levels of the L-gulono-1,4-lactone oxidase (GLO) gene, which is crucial for 2KGA to enhance ASA biosynthesis. The ASA content of GLO-deficient (At5g11540, At5g46750) plants showed no response to exogenous 2KGA treatment. Moreover, changes in the levels of total phenolics and total flavonoids were significantly positively correlated with ASA content. Metabolomics and transcriptomics analyses revealed that the increase in ASA content induced by 2KGA and the subsequent feedback effects influenced major metabolic pathways, including the citric acid cycle, amino acid metabolism, and photosynthesis, as well as varied expression of 30 transcription factors. These findings suggest that exogenous 2KGA has the potential to modulate energy and precursor metabolic pathways by interacting with plant ASA anabolism, thereby facilitating growth and enhancing bioactive compound accumulation. Our study provides novel insights into the role of 2KGA in the biosynthesis of bioactive compounds in plants.
