2026-09-29 理化学研究所
ROSA26-6Rマウスで可視化した、抑制性神経細胞である小脳プルキンエ細胞の微細構造
＜関連情報＞
- https://www.riken.jp/press/2026/20260929_1/index.html
- https://www.cell.com/cell-reports-methods/fulltext/S2667-2375(26)00312-7
Cre、FLP、Dre、Vika、SCre、VCreリコンビナーゼを用いたtdTomatoの強力な標識化を可能にする単一のROSA26マウス系統 A single ROSA26 mouse line for robust tdTomato labeling with Cre, FLP, Dre, Vika, SCre, and VCre recombinases
Toshiaki Nakashiba ∙ Takashi Sugiyama ∙ Yoshitaka Yamashita ∙ … ∙ Kenichi Nakashima ∙ Atsushi Yoshiki ∙ Kuniya Abe
Cell Reports Methods Published:September 28, 2026
DOI:https://doi.org/10.1016/j.crmeth.2026.101611
Highlights
- We develop a single ROSA26-6R mouse line for robust tdTomato labeling by six SSRs
- In vivo profiling shows Vika and VCre are as efficient as the Cre/loxP system
- Bright fluorescence enables 3D whole-embryo and fine-structure imaging
- This multi-recombinase reporter line reduces colony burden for genetic labeling
Summary
Maintaining separate reporter lines for diverse site-specific recombinases (SSRs) imposes a major logistical bottleneck in complex genetic modeling. Here, we developed the ROSA26-6R reporter mouse, a single, universal line providing robust tdTomato expression for six distinct SSRs: Cre, FLP, Dre, Vika, SCre, and VCre. By crossing this line with newly generated CAG-driven SSR driver lines, we established the first systematic in vivo recombination profile for these systems, revealing high recognition specificity for each SSR and identifying Vika and VCre as highly efficient systems comparable to the gold-standard Cre/loxP. The bright native fluorescence in this line enables high-resolution, antibody-free visualization of fine structures, including dendritic spines and axonal trajectories, and facilitates whole-embryo 3D imaging of the developing nervous system. By consolidating responsivity to six SSRs into a single genomic locus, the ROSA26-6R mouse significantly reduces colony maintenance burdens, while providing a versatile, high-performance platform for multi-feature genetic labeling and advanced imaging.
