2026-07-10 マックス・プランク研究所
Artificial cells operating the CETCH cycle, in which active CO2-fixation is shown by green fluorescence.© Max Planck Institute for Terrestrial Microbiology/Ballinger
＜関連情報＞
- https://www.mpg.de/26863300/energy-boost-for-synthetic-cells
- https://www.pnas.org/doi/10.1073/pnas.2613483123
人工呼吸鎖の直接統合による無細胞代謝の改善 Improving cell-free metabolism through direct integration of artificial respiratory chains
Owen D. Jarman, Nitin Bohra, Peter Claus, +1 , and Tobias J. Erb
Proceedings of the National Academy of Sciences Published:July 2, 2026
DOI:https://doi.org/10.1073/pnas.2613483123
Abstract
Energy-conserving mechanisms are essential in supporting cellular life. Yet in synthetic biology, it remains a challenge to reconstruct such processes from the bottom–up and integrate them with other biological functions to create complex systems with life-like properties. Recent efforts to build higher-order cell-free metabolic networks have suffered from the fact that their central oxidation reactions are not coupled to energy conservation, causing kinetic and thermodynamic limitations. Here, we developed an artificial respiratory chain that we tailored to sustain rapid electron transfer in a CO2-fixing 16-enzyme catalytic cycle (crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA), while also exploiting the concurrent electron flow for adenosine triphosphate synthesis. We demonstrate how such artificial respiratory chains can be further diversified to accept multiple electron entries and coupled to other biological functionalities, such as cell-free transcription–translation networks. Altogether, our work highlights the opportunities and challenges of directly integrating energy conservation mechanisms when building toward self-sustaining/self-energizing artificial life-like systems.
