2023-04-24 バース大学
NC細胞は非常に若い胚に存在し、髪や皮膚の色を決定するために不可欠であり、多くの種類の細胞を生み出すことができる。従来は、NC細胞は分化する前に特定の細胞型にコミットすると考えられていたが、この新しい発見により、これらの細胞の可能性が以前にも増して大きくなることが期待される。
NC細胞は多様な細胞に分化する能力を持つため、治療用の細胞としての可能性がある。この発見は、再生医療において重要な意義を持つ。
<関連情報>
- https://www.bath.ac.uk/announcements/developing-cells-likely-can-change-their-mind-about-their-destiny/
- https://www.nature.com/articles/s41467-023-36876-4
ゼブラフィッシュの色素細胞は、持続性のある高度多能性前駆細胞から直接発生する Zebrafish pigment cells develop directly from persistent highly multipotent progenitors
Tatiana Subkhankulova,Karen Camargo Sosa,Leonid A. Uroshlev,Masataka Nikaido,Noah Shriever,Artem S. Kasianov,Xueyan Yang,Frederico S. L. M. Rodrigues,Thomas J. Carney,Gemma Bavister,Hartmut Schwetlick,Jonathan H. P. Dawes,Andrea Rocco,Vsevolod J. Makeev & Robert N. Kelsh
Nature Communications Published:06 March 2023
DOI:https://doi.org/10.1038/s41467-023-36876-4
Abstract
Neural crest cells are highly multipotent stem cells, but it remains unclear how their fate restriction to specific fates occurs. The direct fate restriction model hypothesises that migrating cells maintain full multipotency, whilst progressive fate restriction envisages fully multipotent cells transitioning to partially-restricted intermediates before committing to individual fates. Using zebrafish pigment cell development as a model, we show applying NanoString hybridization single cell transcriptional profiling and RNAscope in situ hybridization that neural crest cells retain broad multipotency throughout migration and even in post-migratory cells in vivo, with no evidence for partially-restricted intermediates. We find that leukocyte tyrosine kinase early expression marks a multipotent stage, with signalling driving iridophore differentiation through repression of fate-specific transcription factors for other fates. We reconcile the direct and progressive fate restriction models by proposing that pigment cell development occurs directly, but dynamically, from a highly multipotent state, consistent with our recently-proposed Cyclical Fate Restriction model.
