2026-08-21 理化学研究所,九州大学,千葉大学,金沢大学,広島大学,北里大学,慶應義塾大学先端生命科学研究所,国立健康危機管理研究機構
本研究の概要
＜関連情報＞
- https://www.riken.jp/press/2026/20260821_3/index.html
- https://link.springer.com/article/10.1186/s40168-026-02476-9
環境プロバイオティクス好熱菌による下痢寛解のための腸管保護代謝表現型 Gut-protective metabolic phenotype for diarrhoeal remission caused by an environmental probiotic thermophile
Hirokuni Miyamoto,Hideyuki Takahashi,Wataru Suda,Haruki Yamano,Yudai Inabu,Hiroaki Kodama,Yumiko Nakanishi,Shigeharu Moriya,Takashi Satoh,Tamotsu Kato,Chie Shindo,Naoko Tsuji,Makiko Matsuura,Chitose Ishii,Teruno Nakaguma,Tetsuji Etoh,Yuji Shiotsuka,Motoaki Udagawa,Atsushi Kurotani,Kenta Suzuki,Hiroshi Masuya,Satoshi Wada,Shinji Fukuda,Yukihiro Tashiro,… Hiroshi Ohno
Microbiome Published:05 August 2026
DOI:https://doi.org/10.1186/s40168-026-02476-9
Abstract
Background
Controlling diarrhoea in humans and livestock is a global challenge with diverse aetiologies. However, the dynamics of the microbiome for diarrhoeal remission are not sufficiently understood, and effective intervention strategies based on environmental microorganisms have not been fully explored.
Methods
We investigated the metabolic structure associated with diarrhoeal remission using a combination of statistical, genomic, and proteomic approaches in a cattle model. Oral administration of the compost-derived thermophile Caldifermentibacillus hisashii significantly ameliorated persistent diarrhoea. Faecal bacterial populations and metabolites were characterised by a multi-step statistical pipeline comprising difference-in-differences (DID) analysis, Cliff’s delta effect size estimation with permutation-based validation. The functional importance of the selected feature components was validated through genomic and proteomic analysis of C. hisashii N11 (AP028807.1).
Results
Oral administration of C. hisashii significantly ameliorated persistent diarrhoea in calves. Although no significant differences in faecal bacterial community composition were observed, integrated analysis of faecal metabolites identified butyrate and 2-aminoisobutyrate (AIB) as the most discriminative features associated with diarrhoeal remission. Genomic and proteomic analyses of C. hisashii confirmed biosynthetic gene clusters for butyrate and AIB-containing lantibiotics, supporting the structural importance of these metabolites in diarrhoeal remission.
Conclusion
These findings suggest that diarrhoeal remission observed in this study involves characteristic shifts in faecal metabolite profiles rather than marked changes in overall gut microbial community composition, highlighting a protective role of C. hisashii as an environmental probiotic against diarrhoeal dysbiosis through modulation of gut microbial metabolic output. This offers a perspective that bridges environmental microbiology and gut health within a One Health framework.
