2026-07-28 理化学研究所
実験植物バイオリソース品質確認の効率化
＜関連情報＞
- https://www.riken.jp/press/2026/20260728_1/index.html
- https://academic.oup.com/pcp/advance-article-abstract/doi/10.1093/pcp/pcag089/8739320
一般的な植物トランスジーンのマルチプレックスPCRに基づく検出方法 Multiplex PCR based detection methods of common plant transgenes
Atsuko Iuchi,Satoshi Iuchi,Yukie Aso,Hiroshi Abe,Masatomo Kobayashi,Taiji Kawakatsu
Plant and Physiology Published:28 July 2026
DOI:https://doi.org/10.1093/pcp/pcag089
Extract
Genetic transformation is a fundamental technique in plant biology and biotechnology, enabling functional gene analysis and the introduction of novel traits into plants and crops. Accurate identification of transgenes is therefore essential for scientific rigor, reproducibility, and transparent resource distribution. Efficient detection of commonly used transgenes is thus critical for genetic verification, biosafety compliance, and reliable sharing of plant resources to ensure research reproducibility. Multiplex PCR combined with capillary electrophoresis is widely used in plant research because of its high sensitivity and throughput, and has also been applied to transgene detection. Recent studies have demonstrated multiplex fluorescent PCR assays capable of detecting dozens of transgenes, mainly targeting trait-associated elements introduced into crop plants for agricultural improvement (Yi et al. 2022). However, such approaches are generally designed for crop- or trait-specific purposes. In contrast, routine research laboratories and stock centers require universal, efficient screening methods targeting widely used selectable markers and reporter genes shared across diverse species and transformation systems, for which optimized high-throughput frameworks remain limited. To address this need, we developed two PCR-based methods for detecting common plant transgenes. Fluorescent detection of transgenes (fDET) enables high-throughput analysis using a single PCR reaction followed by capillary electrophoresis, whereas detection of transgenes (DET) provides a lower-throughput alternative using conventional PCR and agarose gel electrophoresis. Here, we describe the design and performance of fDET as the primary focus of this study.
