2026-04-20 ノースカロライナ州立大学（NC State）
＜関連情報＞
- https://news.ncsu.edu/2026/04/each-epigenome-protein-produces-different-expression/
- https://www.sciencedirect.com/science/article/pii/S2589004226011806
エピゲノム制御因子が単一の真核生物プロモーターに多様な遺伝子発現ダイナミクスを付与する Epigenome Regulators Imbue a Single Eukaryotic Promoter with Diverse Gene Expression Dynamics
Jessica B. Lee, Leandra M. Caywood, Riley Basinger, Lucas Abbott, Nicholas Levering, Albert J. Keung
iScience Available online: 16 April 2026
DOI:https://doi.org/10.1016/j.isci.2026.115805
Graphical abstract
Highlights
- Screened >80 chromatin proteins for diverse dynamic gene expression outputs
- CAPs drive unique delays, production rates, and cell-to-cell variability
- Clustering of dynamics distinguishes coactivators from chromatin remodelers
- A deterministic model captures the full range of observed dynamic profiles
Summary
Biological information can be encoded in signaling dynamics, which have been implicated in many physiological processes; yet the diversity of dynamic expression profiles driven by a single gene remains unclear. To explore this, we screen 80 chromatin associated proteins (CAPs) for their potential to drive diverse dynamic gene expression profiles from the same genome-integrated reporter in yeast. Using locus-specific optogenetic recruitment and live-cell microscopy, we measure dynamic expression profiles within single cells. CAP recruitment elicits a range of responses varying in activation delay, strength, production rate, and noise. We find that promoter activity is characterized by graded, rather than switchlike, transitions. A kinetic model with three promoter states and a positive feedback loop successfully captures the key features of expression driven by each CAP. These results reveal the rich dynamic landscape possible from a single gene, offering insights into native cellular processes and enhancing gene expression control in synthetic biology.
